International Journal of Pharma and Bio Sciences
 
 
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ORIGINAL RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 13 Issue 3, July-September, Pages:16-23

Assessment of Total Phenolic Content, In-Vitro Antioxidant, Anti-Inflammatory and Α-Amylase Inhibitory Activity of Aerial Parts of Alhagimaurorum

Divya Patel, Reena S. Lawrence, Shradhanjali Singh, Amrita raj
DOI: http://dx.doi.org/10.22376/Ijpbs.2022.13.3.p16-23
Abstract:

Alhagimaurorum is a folklore medicinal plant and a member of family Leguminaceae, the present study investigates invitro antioxidant activity, in-vitro anti-inflammatory activity, α- amylase inhibitory activity and total phenolic content from the aerial parts of Alhagi maurorum. Ethanolic extract 50% v/v of the aerial part of Alhagi maurorum(AMEE) was obtained by hot percolation method at 600C. AMEE was subjected for liquid-liquid partitioning to obtain various fractions such as ethyl acetate (AMEA), chloroform (AMCF), methanol (AMMF) and aqueous (AMAF) fractions. The ethanolic extract AMEE and its fraction AMEA were subjected for total phenolic content, preliminary phytochemical screening, TLC-vitro antioxidant activity, in-vitro anti-inflammatory activity and α-amylase inhibitory activity. Folin-Ciocalteu reagent was used to estimate total phenolic content. DPPH was used to determine in-vitro antioxidant activity. Acarbose was used to determine α-amylase inhibition and anti-inflammatory activity of aerial part was investigated by protein denaturation method. Phytochemical analysis of extract and its fraction showed presence of major classes of phytochemicals such as alkaloids, carbohydrates, tannins, flavonoids, steroids. Aerial parts of Alhagi maurorum were found to contain 4.049 mg/g and 4.053 mg/g of Gallic acid equivalent of total phenolic in ethanolic extract and its ethyl acetate fraction respectively. But exhibited moderate antioxidant activity. Determination of the type of α-amylase inhibition by these plant extract and its fraction could provide by successful use of plant chemicals as drug targets. The results showed that the in-vitro antioxidant activity, anti-inflammatory activity and α-amylase inhibitory activity of plant extract and its fraction was 50± 76.8 significant. It was concluded that a vast number of pharmacological and medicinal properties of Alhagi species make these plants a desirable source for development of new drugs.

Keywords: Antioxidant, Anti-inflammatory, ?-amylase inhibitory, DPPH, Total phenolic content, AMEE, AMEA.
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