Alhagimaurorum is a folklore medicinal plant and a member of family Leguminaceae, the present study investigates invitro antioxidant activity, in-vitro anti-inflammatory activity, α- amylase inhibitory activity and total phenolic content from the aerial parts of Alhagi maurorum. Ethanolic extract 50% v/v of the aerial part of Alhagi maurorum(AMEE) was obtained by hot percolation method at 600C. AMEE was subjected for liquid-liquid partitioning to obtain various fractions such as ethyl acetate (AMEA), chloroform (AMCF), methanol (AMMF) and aqueous (AMAF) fractions. The ethanolic extract AMEE and its fraction AMEA were subjected for total phenolic content, preliminary phytochemical screening, TLC-vitro antioxidant activity, in-vitro anti-inflammatory activity and α-amylase inhibitory activity. Folin-Ciocalteu reagent was used to estimate total phenolic content. DPPH was used to determine in-vitro antioxidant activity. Acarbose was used to determine α-amylase inhibition and anti-inflammatory activity of aerial part was investigated by protein denaturation method. Phytochemical analysis of extract and its fraction showed presence of major classes of phytochemicals such as alkaloids, carbohydrates, tannins, flavonoids, steroids. Aerial parts of Alhagi maurorum were found to contain 4.049 mg/g and 4.053 mg/g of Gallic acid equivalent of total phenolic in ethanolic extract and its ethyl acetate fraction respectively. But exhibited moderate antioxidant activity. Determination of the type of α-amylase inhibition by these plant extract and its fraction could provide by successful use of plant chemicals as drug targets. The results showed that the in-vitro antioxidant activity, anti-inflammatory activity and α-amylase inhibitory activity of plant extract and its fraction was 50± 76.8 significant. It was concluded that a vast number of pharmacological and medicinal properties of Alhagi species make these plants a desirable source for development of new drugs.

REFERENCES

1. Wichi HP. Tumor Development by ButylatedHydroxyanisole (BHA) from the Perspective of Effect on fore stomach and Esophageal Squamish Epithelium. Food Chemistry Toxicology, 1988; 26(4):717-723.
2. Pyo Y H, Lee T C, Logendrae L, Rosen R T. Antioxidant Activity and Compounds of Swiss chard (Beta Vulgaris Subspecies Cycis).  Journal of Food Chemistry, 2004;85(1):19-26.Iwe MM. In therapeutic Agents from Ethno medicine. Ethnomedicine and Drug Discovery, Amsierdam, 2002;8(1):69-79.Odunayo RA, Ibukun EA, Tayo A, and Tolu, O.  In vitro antimicrobial activity of crude extract from plants Bryophyllumpinnatum and Kalanchoecrenata. African journal of food Agriculture India, 2007;4(01):31,

1. Yang H, Zhang N, Zeng Q, Yu Q, Ke  S, and Li  X. HPLC method for the simultaneous determination of ten annonaceousacetogenins after super crital fluid CO₂ extraction. International Journal of Biomedicine Sciences, 2010; 6:202–207,
2. Tsai S Y, Huang S J, Lo S H, Wu T P, Lian P Y, Mau J L. Flavor components and antioxidant properties of several cultivated mushrooms.  Journal of Food Chemistry, 2009;113(3):578-584.

1. Ahmad I and Beg AZ. Antimicrobial and phytochemical studies on 45 indian medicinal plants against multidrug resistant human pathogens. Journal of Ethnopharmacology, 2007;74:113-123,
2. Rios JL, Recio MC, Villar A. Screening methods for natural products with antimicrobial activity: a review of the literature. Journal of Ethnopharma, 1988;23:127-149.
3. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 1999;86(6):985-990.
4. Tania MD, Almocida A, Anderia FS, Mitzi B, Telma S, Mesquita G, Etra DF, Smania A, Artur SJ, Crios LZ. Biological screening of Brazilian plants.  Journal of Brazilian Science, 2000;95(3):367-373.
5. Shivashankar M, Rajeshwari S, Nagananda G S, Rajath S, Chandan N. Research pharmaceutical, 2013;3(6):06-13.
6. Shivashankar M, Rajeshwari S, Nagananda G S, Rajath S, Chandan N. Research pharmaceutical, 2013;(6):06-13.
7. Vijaymeena C, Subhanshini G, Loganayagi M, Ramesh B. Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annonamuricata. International Journal of current Microbiology Applied Sciences, 2013;2(1):1-8.
8. Nithiya and Vijayalakshmi.  Antimicrobial activity of fruit extract of Annonasquamosa L.  world journal of pharmacy and pharmaceutical science; 2015;4(5):1257-1267.