The goal of this study is to provide current information on the various secondary metabolite classes as well as experimental evidence of antibacterial activity against a wide range of pathogens. Tinospora cordifolia leaves belong to the family Menispermaceae and identified as heart leaves Moonseed. Invitro antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger was studied by Agar well diffusion method with various concentrations. GC- MS analysis was performed to identify major bioactive compounds present in the extracts. The GC – MS analyses revealed the presence of 25 compounds in the ethanol extract and 25 compounds in the leaf extract composition. The antimicrobial analysis revealed that the extract had a strong inhibitory activity against C.albicans among the pathogens tested, with the highest zone of inhibition being 30 mm at a concentration of 100 mg/ml. Our findings indicate that Tinospora cordifolia leaf extract can be used in traditional medicines to treat a variety of diseases
Keywords: Ethanol extract, Tinospora cordifolia, Antibacterial and antifungal, GC-MS analysis.
Burt S. Essential oils: their antibacterial properties and potential applications in foods – a review. Int J Food Microbiol. 2004;9(3):223-53. doi: 10.1016/j.ijfoodmicro.2004.03.022, PMID 15246235.
Murai Y, Kokubugata G, Yokota M, Kitajima J, Iwashina T. Flavonoids and anthocyanins from six Cassytha taxa (Lauraceae) as taxonomic markers. Biochem Syst Ecol. 2008;36(9):745-8. doi: 10.1016/j.bse.2008.06.007.
Conner DE, Beuchat LR. Effects of essential oils from plants on growth of food spoilage yeasts. J Food Sci. 1984;49(2):429-34. doi: 10.1111/j.1365-2621.1984.tb12437.x.
Ultee A, Bennik MHJ, Moezelaar R. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol. 2002;68(4):1561-8. doi: 10.1128/AEM.68.4.1561-1568.2002, PMID 11916669.
Courtney JL, Lassak EV, Speirs GB. Leaf wax constituents of some Myrtaceous species. Phytochemistry. 1983;22(4):947-9. doi: 10.1016/0031-9422(83)85027-4.
Lassak EV, McCarthy T. Australian medicinal plants. New Holland, Sydney, Australia; 2008.
Palombo EA, Semple SJ. Antibacterial activity of traditional Australian medicinal plants. J Ethnopharmacol. 2001;77(2-3):151-7. doi: 10.1016/s0378-8741(01)00290-2, PMID 11535358.
Helander IM, Alakomi HL, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid EJ, Gorris LGM, von Wright A. Characterization of the action of selected essential oil components on gram-negative bacteria. J Agric Food Chem. 1998;46(9):3590-5. doi: 10.1021/jf980154m.
Huang AC, Wilde A, Ebmeyer J, Skouroumounis GK, Taylor DK. Examination of the phenolic profile and antioxidant activity of the leaves of the Australian native plant Smilax glyciphylla. J Nat Prod. 2013;76(10):1930-6. doi: 10.1021/np4005163, PMID 24050300.
??can G, Kirimer N, Demirci F, Demirci B, Noma Y, Ba?er KHC. Biotransformation of (−)-(R)-α-phellandrene: antimicrobial activity of its major metabolite. Chem Biodivers. 2012;9(8):1525-32. doi: 10.1002/cbdv.201100283, PMID 22899613
Packer J, Naz T, Yaegl Community Elders, Harrington D, Jamie JF, Vemulpad SR. Antimicrobial activity of customary medicinal plants of the Yaegl Aboriginal community of northern New South Wales, Australia: a preliminary study. BMC Res Notes. 2015;8(1):276. doi: 10.1186/s13104-015-1258-x, PMID 26122212.
Packer J, Gaikwad J, Harrington D, Ranganathan S, Jamie J, Vemulpad S. Medicinal plants of New South Wales, Australia. In: Genetic resources, chromosome, engineering and crop improvement. Boca Raton, FL: CRC Press Press; 2012. p. 259-96.
Smyth T, Ramachandran VN, Brooks P, Smyth WF. A study of the antibacterial activities of selected Australian medicinal plants. J Pharmacogn Phytother. 2009;1(6):82-6.
Knobloch K, Pauli A, Iberl B, Weigand H, Weis N. Antibacterial and antifungal properties of essential oil components. J Essent Oil Res. 1989;1(3):119-28. doi: 10.1080/10412905.1989.9697767.
Pennacchio M, Kemp AS, Taylor RP, Wickens KM, Kienow L. Interesting biological activities from plants traditionally used by Native Australians. J Ethnopharmacol. 2005;96(3):597-601. doi: 10.1016/j.jep.2004.10.012, PMID 15619584.
Jarvis LM. Imminent threat. Chem Eng News Archive. Chemical and Engineering News. 2008;86(15):22-4. doi: 10.1021/cen-v086n015.p022..
Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol. 2001;91(3):453-62. doi: 10.1046/j.1365-2672.2001.01428.x, PMID 11556910.
McMeniman E, Holden L, Kearns T, Clucas DB, Carapetis JR, Currie BJ, Connors C, Andrews RM. Skin disease in the first two years of life in Aboriginal children in East Arnhem Land. Australas J Dermatol. 2011;52(4):270-3. doi: 10.1111/j.1440-0960.2011.00806.x, PMID 22070701.
Naz T, Packer J, Yin P, Brophy JJ, Wohlmuth H, Renshaw DE, Smith J, Elders YC, Vemulpad SR, Jamie JF. Bioactivity and chemical characterisation of Lophostemon suaveolens—an endemic Australian Aboriginal traditional medicinal plant. Nat Prod Res. 2016;30(6):693-6. doi: 10.1080/14786419.2015.1038260, PMID 25942679.
Oosterhaven K, Poolman B, Smid EJ. S-carvone as a natural potato sprout inhibiting, fungistatic and bacteristatic compound. Ind Crops Prod. 1995;4(1):23-31. doi: 10.1016/0926-6690(95)00007-Y.
Packer J, Brouwer N, Harrington D, Gaikwad J, Heron R, Yaegl Community Elders, Ranganathan S, Vemulpad S, Jamie J. An ethnobotanical study of medicinal plants used by the Yaegl Aboriginal community in northern New South Wales, Australia. J Ethnopharmacol. 2012;139(1):244-55. doi: 10.1016/j.jep.2011.11.008, PMID 22101358.
Sen CK, Khanna S, Gordillo G, Bagchi D, Bagchi M, Roy S. Oxygen, oxidants, and antioxidants in wound healing: an emerging paradigm. Ann N Y Acad Sci. 2002;957(1):239-49. doi: 10.1111/j.1749-6632.2002.tb02920.x, PMID 12074976.
Setzer WN, Setzer MC, Bates RB, Jackes BR. Biologically active triterpenoids of Syncarpia glomulifera bark extract from Paluma, North Queensland, Australia. Planta Med. 2000;66(2):176-7. doi: 10.1055/s-2000-11129, PMID 10763596.
Shin S, Kang CA. Antifungal activity of the essential oil of Agastache rugosa Kuntze and its synergism with ketoconazole. Lett Appl Microbiol. 2003;36(2):111-5. doi: 10.1046/j.1472-765x.2003.01271.x, PMID 12535132.
Sikkema J, de Bont JA, Poolman B. Interactions of cyclic hydrocarbons with biological membranes. J Biol Chem. 1994;269(11):8022-8. doi: 10.1016/S0021-9258(17)37154-5, PMID 8132524.
Sikkema J, de Bont JA, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev. 1995;59(2):201-22. doi: 10.1128/mr.59.2.201-222.1995, PMID 7603409.
Silva NCC. Estudo comparativo da ação antimicrobiana de extratos e óleos essenciais de plantas medicinais e sinergismo com drogas antimicrobianas [thesis]. Botucatu: Universidade Estadual Paulista, Instituto de Biociências. 67 p; 2010.
Thoroski J, Blank G, Biliaderis C. Eugenol induced inhibition of extracellular enzyme production by Bacillus cereus. J Food Prot. 1989;52(6):399-403. doi: 10.4315/0362-028X-52.6.399, PMID 31003298.
Van Vuuren SF, Suliman S, Viljoen AM. The antimicrobial activity of four commercial essential oils in combination with conventional antimicrobials. Lett Appl Microbiol. 2009;48(4):440-6. doi: 10.1111/j.1472-765X.2008.02548.x, PMID 19187494.
Wendakoon CN, Sakaguchi M. Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Prot. 1995;58(3):280-3. doi: 10.4315/0362-028X-58.3.280, PMID 31137282.
Zago JAA, Ushimaru PI, Barbosa LN, Fernandes Jr A. Synergism between essential oils and antimicrobial drugs against Staphylooccus aureus and Escherichia coli strains from human infections. Rev Bras Pharmacogn. 2009;19(4):828-33.
Zielins ER, Brett EA, Luan A, Hu MS, Walmsley GG, Paik K, Senarath-Yapa K, Atashroo DA, Wearda T, Lorenz HP, Wan DC, Longaker MT. Emerging drugs for the treatment of wound healing. Expert Opin Emerg Drugs. 2015;20(2):235-46. doi: 10.1517/14728214.2015.1018176, PMID 25704608.
