International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 12 Issue 2, 2021 (April-June), Pages:55-63

Investigations on Antibacterial Potency of Azadirachta Indica Leaf Extracts against Some Resistant Bacteria

N. B. Awandekar , Kalyani Game and M. J. Umekar

Resistance to antibiotics in pathogens responsible for causing infectious diseases has become a matter of great public health concern internationally and India is no exception to it. Widespread use and availability of practically all the antimicrobials across the counter and incompletion of the dose is supposed to be one of the major reasons for this. It is observed that some plant extracts seem to potentiate the activity of antibiotics against resistant bacterial strains. So the objective of the study was to use some natural products as an alternate to the conventional treatment against antibiotic resistance. The effects of crude extracts of leaves of Azadirachta indica against resistant strains of pathogenic S. aureus, E. coli and K. Pneumoniae were investigated using the agar well diffusion method. It was observed that the growth of all the isolates were inhibited to varying degrees with gram-positive more susceptible than gram-negative bacteria. Hexane extract was more effective, producing larger zones of growth inhibition sizes and smaller MIC, than the aqueous and ethyl acetate extracts. The MIC values ranged from 0.5 - 4 mg/ml. The ability of the crude extracts to inhibit the growth of such resistant pathogenic bacterial strains that were used in this study is an indication that the neem leaves extract has the potential and can be used as a source for new broad spectrum oral antibiotics. The result obtained in this study validates the use of the neem leaves extract in traditional medicine to treat infectious conditions.

Keywords: Antibiotic, Resistance, Neem extracts, MIC
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1.       Srivastava RK. National policy for containment of antimicrobial resistance India; Directorate General of Health Services. New Delhi: Ministry of Health & Family Welfare Nirman Bhawan; 2011. p. 5-8.

2.       Bassetti M, Merelli M, Temperoni C, Astilean A. New antibiotics for bad bugs: where are we? Ann Clin Microbiol Antimicrob. 2013;12:22. doi: 10.1186/1476-0711-12-22, PMID 23984642.

3.       Iwu Maurice M, Duncan Angela R, Chris O. O. New antimicrobials of Plant Origin; Perspectives on new crops and new uses Janick J, editor. Vol. 1999. Alexandria, VA: ASHS Press. p. 457-62.

4.       Sabahat S, Perween T. Antimicrobial activities of Emblica officinalis and Coriandrum sativum against gram positive bacteria and Candida albicans. Pak J Bot. 2007;39(3):913-7.

5.       Hamilton-Miller JMT. Antibiotic resistance from two perspectives: man and microbe. Int J Antimicrob Agents. 2004;23(3, March):209-12. doi: 10.1016/j.ijantimicag.2003.12.001, PMID 15164959.

6.       Costa D, Poeta P, Sáenz Y, Coelho AC, Matos M, Vinué L, Rodrigues J, Torres C. Prevalence of antimicrobial resistance and resistance genes in faecal Escherichia coli isolates recovered from healthy pets. Vet Microbiol. 2008;127(1-2):97-105. doi: 10.1016/j.vetmic.2007.08.004, PMID 17870255.

7.       Takarada K, Kimizuka R, Takahashi N, Honma K, Okuda K, Kato T. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol. 2004 February;19(1):61-4. doi: 10.1046/j.0902-0055.2003.00111.x, PMID 14678476.

8.       Oluwatuyi M, Kaatz GW, Gibbons S. Antibacterial and resistance modifying activity of Rosmarinus officinalis. Phytochemistry. 2004 December;65(24):3249-54. doi: 10.1016/j.phytochem.2004.10.009, PMID 15561190.

9.       BiswasIshita CRK, BanerjeeUday Bandyopadhyay K. Biological activities and medicinal properties of Neem (Azadirachta indica). Curr Sci. November 2001;82(11).

10.     Schmutterer H. The neem tree: Azadirachta indica A. Juss. and other meliaceous plants. Weinheim: VCH Verlagsgesellschaft mbH; 1995. p. 1-34.

11.     Isman MB, Koul O, Arnason JT, Stewart J, Salloum GS. Developing a neem-based insecticide for Canada. Mem Entomol Soc Can. 1991;123(S159):39-46. doi: 10.4039/entm123159039-1.

12.     AkulaAnisha AkulaAnisha ARDC. Somatic embryogenesis in clonal neem, Azadirachta indica A. Juss. and analysis for in vitro azadirachtin production, vitro cellular & Developmental Biology – Plant. 2003;39(3):304-10.

13.     Baker CN, Thornsberry C, Hawkinson RW. Inoculum standardization in antimicrobial susceptibility testing: evaluation of overnight agar cultures and the rapid inoculum standardization system. J Clin Microbiol. 1983;17(3):450-7. doi: 10.1128/JCM.17.3.450-457.1983, PMID 6841581. PMCID PMC272664.

14.     Weinstein Melvin P, Patel Jean B, Bobenchik April M et al. Performance standards for antimicrobial susceptibility testing. 29th ed. Vol. M100. Clinical and Laboratory Standards Institute; 2019.

15.     Sahm DF, Washington JA, JA. Antibacterial susceptibility test dilution methods. In: Manual of clinical Microbiology. Lennette E.H. 5th ed Am.Soc.Microbiol. Washington DC; 1990. p. 1105-16.

16.     Hugo SB, Rusell AD. Pharmaceutical microbiology. 8th ed; 2011. Blackwell W. Publication: A john Wiley & Sons, Ltd. London pp.217-30 & 293-311.

17.     White DG, Acar J, Anthony F, Franklin A, Gupta R, Nicholls T, Tamura Y, Thompson S, Threlfall EJ, Vose D, Van Vuuren M, Wegener HC, Costarrica ML, Office International des Epizooties Ad hoc Group. Antimicrobial resistance: Standardisation and harmonisation of laboratory methodologies for the detection and quantification of antimicrobial resistance. Rev Sci Tech. 2001;20(3):849-58. doi: 10.20506/rst.20.3.1316, PMID 11732427.

18.     Salman MT, Khan RA, Shukla I. Antimicrobial activity of Nigella sativa Linn. seed oil against multi-drug resistant bacteria from clinical isolates. Nat Prod Radiance. 2008;7(1):10-1.

19.     Tijani Y, Uguru MO, Salawu OA. Anti-pyretic, anti-inflammatory and anti-diarrhoeal properties of Faidherbia albida in rats. Afr J Biotechnol. 2008;7(6):696-700.

20.     Vermani K, Garg S, Zaneveld LJ. Assemblies for in vitro measurement of bioadhesive strength and retention characteristics in simulated vaginal environment. Drug Dev Ind Pharm. 2002;28(9):1133-46. doi: 10.1081/ddc-120014580, PMID 12455472.

21.     Mohammed HA, AlFadhil AO. Antibacterial activity of Azadirachta indica (Neem)leaf extract against bacterial pathogens in Sudan. Am J Res Commun. 2015;3(5):246.

22.     Raut Ranjit R, Sawant Ajit R, Jamge Bhagyashree B. Antimicrobial activity of Azadirachta indica (Neem) against Pathogenic microorganisms. J Acad Ind Res (JAIR);3(7, December):327-9.

23.     El-Mahmood AM, Ogbonna OB, Raji M. The antibacterial activity of Azadarichta indica (neem) seeds extracts against bacterial pathogens associated with eye and ear infections. J Med Plants Res. 2010;4(14):1414-21.

24.     Muhammad A, Lawan IM, Abubakar A, Dangora II. Antimicrobial activity of Azadirachta indica (Neem) leave extract against some clinical isolates, DUJOPAS. 2019;5(1b):97-104.

25.     Nwakaeze et al., 2013. Emmanuel N, Ifeanyichukwu I, Chika E, Emmanuel E, Chinwe N. Inhibitory effects of Neem (Azadirachta indica Linn.) and Bitter Kola (Garcinia kola Heckel) leaves on selected pathogenic bacteria, Academic Journals. Vol. 7(41); November 8, 2013. p. 2763-7.

26.     Akujobi C, Anyawu BN, Onyeze C, Ibekwe. Antibacterial activities and preliminary screening of four medical plants. J Appl Sci. 2004;7(3):4328-38.

27.     Ogbulie JN, Ogueke CC, Okoli LO, Anyawu BN. Antibacterial activities and toxicological potential of crude ethanolic extracts of Euphorbia hirta. J Afr J Biotechnol. 2007;6(13):1544-8.

28.     Mohammed M, Pateh UU, Maika NO, lami L, Abdulwaliyu I. Phytochemical and antimicrobial activities of the leaf extract of Stachytarpheta angustifolia (Mill.) Vahl Verbenaceae. Int J Sci Eng Technol. 2012;2(10).


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