International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 12 Issue 2, 2021 (April-June), Pages:60-70

Study on Characteristic Separation, Identification and Antimicrobial Activity of Main Compounds from the Rhizomes of Alpinia Allughas.

Nikhil Kumar, Vijayata and Shilpi Sharma

Alpinia allughas Rosc. is a Zingiberace herb has been used in this study. A systematic study has been done for the chemical constituents of this plant and bioactive compounds responsible for the medicinal properties were identified by the chemical examination of rhizome extract. Also the antimicrobial properties of the active compounds has also been studied. To carryout the studies the extract of crushed dried rhizomes of A. allughas was partitioned with chloroform solvent (Hexane, CHCl3, EtOAc, BuOH) system. Fractions are subjected to column chromatography on silica gel or alumina. Column was packed in pure petroleum ether and ran with increasing percentage of ethyl acetate as eluent. The compounds were fractionated and isolated from petroleum ether and ethyl acetate  with different  polarities  1%, 2% 3% 4%, 5%, 8%,10% increasing order with help of  column chromatography and thin layer chromatographic technique. The chloroform extract of rhizomes of Alpinia allughas showed the presence of five prominent and a number of other spots on thin layer chromatography (TLC). All the fractions were collected and distilled over water bath. TLC of each fraction was regularly checked in various combinations of petroleum ether-ethyl acetate and chloroform-methanol. Compounds A, B, and C were isolated at different combinations of petroleum ether and ethyl acetate on silica gel for that the column extract was subjected to column chromatography over silica gel. Elemental analysis were carried out for each extracted compounds followed by various spectral analysis including Infra red, 1H NMR, 13C NMR and mass spectroscopy. Finally the antimicrobial properties of all the active compounds extracted was studied against the three different bacterial strains.

Keywords: Alpinia allughas Rosc, Zingiberaceae Column Extraction Method, Column Chromatography, Isolation Compounds, Spectroscopy Technique.
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1.       Bhattacharjee SK. Hand book of medicinal plants. Jaipur: Pointer Publishers; 1998. p. 3-4.

2.       Phytochemistry, standardisation and biotechnological aspects of ISM drugs, W.H.O. Ghaziabad: Training, Cum-Workspop, Pharmacopoeial Laboratory for Indian Medicine; 2001. p. 37-8.

3.       Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants CSIR. New Delhi; 1956. p. 13-4.

4.       Kirtikar KR, Basu BD. Indian medicinal plants. Lalit Mohan Basu Allahabad. 1970;4:3381.

5.       Gupta AK, Tandon N. Reviews on Indian medicinal plants. Vol. 2. New Delhi: indian council of medical research; 2004. p. 120-1.

6.       Goswami T, Saikia CN. Evaluation of properties of fibers extracted from certain weeds for use in paper and cordage industries. J Sci Ind Res. 2003;62:802.

7.       Sailia CN, Ali F, Ghosh SR. Evaluation of properties of fiber extracted from certain weed for use paper and cordage industries. Adv Fores Res. 1996;14:66.

8.       Kariyone T, Matsushima Y. The essential oil of Zingiber nigrum. Yakugaku Zasshi. 1927;47:674-6.

9.       Purohit RM, Devi K. Chromatographic analysis of the essential oil from of Alpinia allughas. riechst aromen koerperflegem. 1976;26(7):139-2.

10.     Prakash O, Joshi S, Pant AK, Chanotiya CS, Mathela CS. Volatile constituents of rhizomes and leaves of Alpinia allughas roscoe. J Essent Oil Res. 2007;19(5):407-9. doi: 10.1080/10412905.2007.9699937.

11.     Chunfeng Q, Zhengtao W, Hui D, Luoshan X, Xiaojiang H. The chemical constituents of blackfruit galangal (Alpinia nigra). Zhongcaoyao. 2000;31:404.

12.     Borthakur SK. Postnatal care of women in traditional system in Assam. Ethnobot. 1996;8:51.

13.     Choudhury MD, Shil S. Antibacterial activity of some athnomedicinal plants. Ann Biol. 2005;87(21):187-2.

14.     Roy B, Tandon V. Flukicidal activity of Alpinia nigra (Zingiberaceae) against the tremtode, Fasciolopsis buski. in humans. Biol med [lett:1999];60:23-9.

15.     Chunfeng Q, Luashan X, Zhengtao W. Pharmacognostical studies on the rhizomes and root of Alpinia nigra. J Chin Med Mater. 2001;24(7):486-7.

16.     Vankar PS, Tiwari V, Singh LW, Swapana N. Electron J Environ Agric Food Chem. 2006;5:1318.

17.     Chirangini P, Sharma GJ, Sinha SK. Sulfur free radical reactivity with curcumin as reference for evaluating antioxydant prophéties of médicinal Zingiberales. J Environ Pathol Toxicol Oncol. 2004;23(4):227-6.

18.     Forgo P, Kover KE. Triterpenoids and steroids isolated from aerial parts of Glochidion multiloculare. 2005;69(43):163-5.

19.     Habsah M, Lajis NH, Ali AM, Sukari MA, Hin YY, Kikuzaki H, Nakatani N. The antioxidative components from Alpinia nutans. Pharm Biol. 2003;41(1):7-9. doi: 10.1076/phbi.

20.     Tram LH, Giang PM, Son PT. A literature-based phyto-pharmacological review on A. conchigera Griff. J Chem. 2007;45:260-1.

21.     Clouse SD. Molecular genetic analysis of brassinosteroid action. Plant J. 1997;10:1-2. doi: 10.1111/j.1399-3054.1997.tb03077.x.

22.     Tilvis RS, Miettinen TA. Serum plant sterols and their relation to cholesterol absorption. Am J Clin Nutr. 1986;43(1):92-7. doi: 10.1093/ajcn/43.1.92, PMID 3942097.

23.     Gupta MB, Nath R, Srivastava N, Shanker K, Kishor K, Bhargava KP. Anti-inflammatory and antipyretic activities of beta-sitosterol. Planta Med. 1980;39(2):157-63. doi: 10.1055/s-2008-1074919, PMID 6967611.

24.     Bhattacharyya AK, Connor WE, Lin DS. The origin of plant sterols in the skin surface lipids in humans: from diet to plasma to skin. J Invest Dermatol. 1983;80(4):294-6. doi: 10.1111/1523-1747.ep12534670, PMID 6833786.

25.     Klippel KF, Hiltl DM, Schipp B. A multicentric, placebo-controlled, double-blind clinical trial of beta-sitosterol (phytosterol) for the treatment of benign prostatic hyperplasia. German BPH-Phyto Study group. Br J Urol. 1997;80(3):427-32. doi: 10.1046/j.1464-410X.1997.t01-1-00362.x, PMID 9313662.

26.     Von Holtz RL, Fink CS, Awad AB. β ? sitosterol activates the sphingomyelin cycle and induces apoptosis in LNCaP human prostate cancer cells. Nutr Cancer. 1998;32(1):8-12. doi: 10.1080/01635589809514709, PMID 9824850.

27.     Kosuge T, Yokota M, Sugiyama K, Saito M, Iwata Y, Nakura M, Yamamoto T. New 1, 4-dihydropyridine derivatives with potent and long-lasting hypotensive effect. Chem Pharm Bull. 1985;33:3565-7.

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