Searching for novel microorganisms and accurate assessment of the steps are essential approaches to trim the costs of disparate processes using industrially important enzymes. This study was aimed at the exploration of various culture parameters involving production of cellulase (exo and endo-1,4,β-D-glucanases) using fungal culture purpureocillium lilacinum by shake flasks culture method. Optimum pH, temperature, incubation time, inoculum volume and agitation rate was revealed to be 5.5, 30° C, 7 days, 2 ×10 ⁶ spores/ml and 150 rpm respectively. Various carbon sources used in this study, 3 % xylose (w/v) was proved to be the best source for maximum FPase (Filter paperase-1.15 IU/ml) and CMCase (carboxy-methyl cellulase-2.09 IU/ml) production. Ammonium sulphate with 0.2% (w/v) showed higher titres of FPase (1.11 IU/ml) and CMCase (1.90) IU/ml) production compared to the remaining nitrogen sources. Addition of Tween-80 at 0.02% (v/v) to the fermentation medium favoured the production of FPase (1.14 IU/ml) and CMCase (1.77 IU/ml) compared to rest of the non-ionic surfactants.