The current study used the culturable endophytic bacterium Nocardiopsis sp. BER-17 isolated from the barks of Cotylelobium melanoxylon to synthesize a novel antimicrobial compounds. Cotylelobium melanoxylon is one of therapeutic plant species used in traditional medicine. It is containing compounds with antibacterial activity and bacterial endophytes that provide bioactive metabolites for medicinal purposes, and sources of novel compounds. An attempt has been made to employ endophytic bacterium isolate from medicinal plants in the synthesis of antibacterial compounds. The objectives of this study was to investigate the antibacterial activities produced by Nocardiopsis sp. BER-17. Dichloromethane endophytic bacterial extract and culture broth were tested for antimicrobial activity using well diffusion method against pathogenic bacteria of Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 25923, Bacillus subtilis FNCC 0061 and Pseudomonas aeruginosa FNCC 0063. The antibacterial activities were assessed based by the presence of clear zones as inhibition zones. Composition of secondary metabolites in the dichloromethane bacterial extract was spectrometrically analyzed using GC-MS. The endophytic bacterial extract activity produced with clear zones against both Gram negative and Gram positive bacterial pathogens. Based on GC-MS result, dichloromethane extract endophytic contain components antibacterial activity.