Int J Pharm Bio Sci Volume 1 Issue 3, 2010 (July - September), Pages:-
Effect of the Different Auxines and Cytokinins in Callus Induction, Shoot, Root Regeneration in Sugarcane
K. Gopitha,A. Lakshmi Bhavani,J. Senthilmanickam
DOI:
Abstract:
Studies on micropropogation of callus culture was undertaken. In which expalnts were inoculated in MS medium fortified with various concentration of 2,4-D, auxin, cytokinin, sucrose at different pH level .The best callus induction was observed at 3.0mg/L, 2,4-D with 10% coconut milk (CM). Best regeneration of shoot was achieved when they were cultured on MS medium supplemented with BAP 1.0mg/L and IBA 0.5mg/L. Among the different media tested with 3mg/L NAA and 5% sucrose supplemented media proved best production of roots.
Keywords: MS medium –Murashige and skoog medium BAP - 6-Benzyl amino purine; Kn - kinetin: IBA- Indole-3-Butyric acid: NAA -Napthalene acetic acid: CM - coconut milk