International Journal of Pharma and Bio Sciences
 
 
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ORIGINAL RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 1 Issue 2, 2010 (April - June), Pages:-

Molecular Characterization of Morphologically Similar Four Pieridae Butterflies (Lepidoptera: Insecta) by RAPD-PCR Technique

Ashish D. Tiple,Sonali V. Padwad,Vishal P. Deshmukh
DOI:
Abstract:

Molecular characterization of four morphologically similar Pieridae butterflies was carried out using RAPD markers. Total 121 bands were scored with 8 decamer primers, of which 115 were polymorphic while 6 were monomorphic and the percentage of polymorphism was 95.04%. Dendrogram based on average similarity coefficient of 08 primers grouped four Pieridae species into two distinct clusters. Cluster-I comprised of 3 species viz. Eurema laeta, E. hecabe and E. blanda, while cluster-II consisted of E. andersonii. Cluster-I further subdivided into two sub-clusters, sub-cluster-I consisting of only Eurema laeta and sub-cluster-II comprised of E. hecabe and E. blanda. Genetic similarities between these four species were discussed on the basis of morphological characters and RAPD data. 

Keywords: Butterflies, Genetic diversity, Molecular characterization, Pieridae, RAPD
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