The medicinal plant Gymnema sylvestre is famous for its antidiabetic potential in the herbal world is concerned. The Macroscopic, Physiochemical parameters, phytochemical screenings were carried out to facilitate quick identification and selection of the drug from various adulterants. The extracts prepared using successive solvent extraction techniques were screened for its antimicrobial activity by Agar well diffusion method against Streptococcus mutans, Staphylococcus aureus, Streptococcus mitis and Candida albicans with the doses 25, 50 and 100 mg/ml. The methanol extract showed strong antimicrobial activity with the zone of inhibition ranges from 12-23mm at 25mg/ml. The successive extracts of Gymnema sylvestre R.Br. was screened for its particle size, total microbial load, investigation with GC-MS and HPTLC studies.  The Gymnema sylvestre hydro alcoholic extract and paste base, tooth powder base were used in the formulation of   “Gurmar Herbal tooth paste” and “Gurmar Herbal Tooth powder”   results found to be within the limits. This proves that the extract can be useful to treat the dental caries with the scientific documentation.