The unique property of the silver nanoparticles having the antimicrobial activity gained the major attention towards the present nanotechnology. Green synthesis of silver nanoparticles (AgNPs) was performed from aqueous silver nitrate using Calotropis procera leaves (L) and stem (S) extract. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The XRD analysis shows that the AgNPs are of face centered cubic (FCC) structure and size was ranging from 19 to 45 nm (L) and 26 to 38 nm (S) respectively. Scanning electron microscopy (SEM) analysis showed that the synthesized AgNPs have spherical shape. The silver nanoparticles possess significant antimicrobial potential against K. pneumonia and S. typhi and also showed good antioxidant property.