Central administration of colchicine is well known to cause cognitive impairment and oxidative damage, which simulates sporadic dementia of the Alzheimer type in humans. Berberine, a natural product alkaloid, has been shown to display a wide array of pharmacological effects. The present study has been designed to investigate the effects of berberine against the colchicine-induced cognitive impairment in mice. Memory performance task was accessed using Morris water maze. Colchicine (15µg/5µL) was administered ICV, resulted in poor memory retention in the Morris water maze task. Mice received chronic treatment of four doses (5, 10, 20, 40mg/kg per day, PO) of berberine for a period of 21 days beginning 4 days prior to colchicine administration. Berberine treatment significantly ameliorated learning deficits, long-term spatial memory retention. Our results suggest that berberine provides antiamnesic effects in colchicines induced memory impairment model and promising agent for the treatment of AD.