METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ATAZANAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS AND ITS STRESS DEGRADATION STUDIES USING UV-VIS SPECTROPHOTOMETRIC METHOD

Authors

  • Suddhasattya Dey Guru Nanak Institute of Pharmacy, Ibrahimpatnam,Hyderabad, INDIA.
  • Y.Vikram Reddy Guru Nanak Institute of Pharmacy, Ibrahimpatnam,Hyderabad, INDIA.
  • Thirupathi Reddy Guru Nanak Institute of Pharmacy, Ibrahimpatnam,Hyderabad, INDIA.
  • Sudhir Kumar Sahoo Royal college of Pharmacy and health Sciences, Berhampur, Orissa, INDIA.
  • P.N. Murthy Royal college of Pharmacy and health Sciences, Berhampur, Orissa, INDIA.
  • Subhasis Mohapatra Royal college of Pharmacy and health Sciences, Berhampur, Orissa, INDIA.
  • S. Subhasis Patro Royal college of Pharmacy and health Sciences, Berhampur, Orissa, INDIA.

Keywords:

Atazanavir, HIV, λmax, ICH, UV-VIS spectroscopy.

Abstract

The present study describes a simple, accurate, precise and cost effective UV-VIS Spectrophotometric method for the estimation of Atazanavir, an anti-HIV drug, in bulk and pharmaceutical dosage form. The solvent used was methanol and the λmax or the absorption maxima of the drug was found to be 250nm. A linear response was observed in the range of 1050µg/ml with a regression coefficient of 0.999. The method was then validated for different parameters as per the ICH (International Conference for Harmonization) guidelines.  This method can be used for the determination of Atazanavir in quality control of formulation without interference of the excipients. Atazanavir sulphate was subjected to stress degradation under different conditions recommended by ICH. The samples so generated were used for degradation studies using the developed method. 
 

Downloads

Published

30.09.2010

How to Cite

Suddhasattya Dey, Y.Vikram Reddy, Thirupathi Reddy, Sudhir Kumar Sahoo, P.N. Murthy, Subhasis Mohapatra, & S. Subhasis Patro. (2010). METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ATAZANAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS AND ITS STRESS DEGRADATION STUDIES USING UV-VIS SPECTROPHOTOMETRIC METHOD. International Journal of Pharma and Bio Sciences, 1(3), 1–8. Retrieved from https://ijpbs.net/index.php/journal/article/view/95

Issue

Volume 1, Issue 3, 2010

Section

Research Articles