Determination Of Auxotrophic Nature Of The mutant Micrococcus Glutamicus Ab100

Abstract

An experimental study was carried out to investigate the auxotrophic nature of the newly developed mutant Micrococcus glutamicus AB₁₀₀. To investigate the role of biotin on growth and l-glutamic acid production by this strain, yeast extract from the minimal salt medium was replaced by 0.2 µg/ml of biotin. Biotin (0.2 µg/ml) was proved to be an essential element for growth production was decreased significantly (p<0.01). But excess biotin created over growth, leading to anaerobic condition which could adversely affect l-glutamic acid production. Thus, in this present study, it was proved that this strain a biotin auxotroph.