DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF Β-SITOSTEROL AND LUPEOL IN VERNONIA CINEREA LINN.

Authors

  • WILLY SHAH* Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai-400 019.
  • M.B.KEKARE Department of Chemistry, Kirti College, Dadar, Mumbai-400 028.
  • VIKAS VAIDYA Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai-400 019.

Keywords:

HPLC, β-sitosterol, Lupeol, Vernonia cinerea Linn.

Abstract

A simple, precise and accurate high-performance liquid chromatographic method has been developed for simultaneous determination of β-sitosterol and Lupeol in whole plant powder of Vernonia cinerea Linn. Chromatographic separation of the two compounds was performed on a waters symmetry shield C18 column (150 x 4.6, 5 µm) as stationary phase with a mobile phase comprising of methanol : acetonitrile (30:70) v/v at a flow rate of 1.0 mL min-1 and UV detection at 210nm with a run time of 12.0 min. The proposed method was validated for linearity, accuracy, precision and limit of quantitation. The validated HPLC method can be used for a routine quality control analysis and simultaneous quantitation of β-sitosterol and Lupeol from Vernonia cinerea Linn. 

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Published

30.09.2010

How to Cite

WILLY SHAH, M.B.KEKARE, & VIKAS VAIDYA. (2010). DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF Β-SITOSTEROL AND LUPEOL IN VERNONIA CINEREA LINN. International Journal of Pharma and Bio Sciences, 1(3), 1–5. Retrieved from https://ijpbs.net/index.php/journal/article/view/93

Issue

Volume 1, Issue 3, 2010

Section

Research Articles