A Kinetic Study On Cellulase Enzymes From Aspergillus Niger

Authors

  • SAXENA ARTI Deptt. of Chemistry , A.N.D.N.N.M.College, Kanpur.
  • SAXENA ANURAG Deptt. of Physics, D.A-V. College,Kanpur.
  • YADAV SARIKA Deptt. of Chemistry , A.N.D.N.N.M.College, Kanpur.

Keywords:

Biodegradation, fungicides, inhibitive

Abstract

A physico-chemical study was made on cellulase enzymes from a highly cellulolytic fungus Aspergillus niger. Various kinetic parameters were estimated by making use of potent fungicides, viz. m-dinitrobenzene and pentachlorophenol. The enzyme showed maximum activity at pH 5.0 and temp. 55o C. The half-life was found to be 42 minutes at optimum pH. The Michaelis Constant, Km, was observed as 13.3mM and Vmax. as 6.65µM/sec. The inhibition constant, Ki, for MDB was observed to be 19.2mM and for PCP as 22.6mM, thereby indicating that MDB is a more potent inhibitor than PCP.

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Published

30.09.2011

How to Cite

SAXENA ARTI, SAXENA ANURAG, & YADAV SARIKA. (2011). A Kinetic Study On Cellulase Enzymes From Aspergillus Niger. International Journal of Pharma and Bio Sciences, 2(3), 37–41. Retrieved from https://ijpbs.net/index.php/journal/article/view/929

Issue

Volume 2, Issue 3, 2011

Section

Research Articles

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