Isolation And Amplification Of Fem – A Gene From Mrsa Isolates

Authors

  • S. GANESH MANIKANDAN Department of Biotechnology, V.MK.V.Engineering College, Vinayaka Mission University, India
  • M. HEMALATHA School of Biological Sciences, PRIST University, Vallam, India.
  • C. LAKSHMINARASIMHAN School of Biological Sciences, PRIST University, Vallam, India.
  • N. THAJUDDIN Department of Microbiology, Bharathidasan University, Thiruchirappalli, India.

Keywords:

Methicillin Resistant Staphylococcus aureus, LB Agar,Real Time PCR, Fem A gene, Agarose Gel Electrophoresis.

Abstract

Statphylococcus aureus is a Gram positive bacterium, which is naturally present in nasopharyngeal region of human beings and animals. After 1941, S.aureus was developed drug resistant capacity to against all penicillin antibiotics. In 1961, Methicillin Resistant Staphylococcus aureus were isolated. The present study Methicillin Resistant S. aureus were isolated from clinical pus samples. All isolates were grown on Luria Bertani (LB) Agar containing Oxacillin antibiotic. Genomic DNA isolated from isolates using with standard procedure. Fem A gene amplification using with Real Time PCR and suitable primers. The amplified gene was produced 510 bp at Agarose Gel Electrophoresis.

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Published

30.09.2011

How to Cite

S. GANESH MANIKANDAN, M. HEMALATHA, C. LAKSHMINARASIMHAN, & N. THAJUDDIN. (2011). Isolation And Amplification Of Fem – A Gene From Mrsa Isolates. International Journal of Pharma and Bio Sciences, 2(3), 29–36. Retrieved from https://ijpbs.net/index.php/journal/article/view/928

Issue

Volume 2, Issue 3, 2011

Section

Research Articles

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