Genetic Characterization Of Bactrocera (Dacus) Flies (Diptera: Tephritidae) Based On RAPD-PCR

Authors

  • R. SINGH Department of Zoology, University of Allahabad, Allahabad, 211002, India
  • N. BAJPAI Department of Zoology, University of Allahabad, Allahabad, 211002, India
  • R. R. TEWARI Department of Biotechnology, School of Life Sciences, Vels University, Chennai, India

Keywords:

RAPD-PCR, Bactrocera, molecular markers, heterozygosity, genetic identity.

Abstract

Genetic characterization of two species of fruit flies belonging to the genus Bactrocera (=Dacus), namely, B. cucurbitae and B. dorsalis was carried out using ten RAPD markers. In B. cucurbitae and B. dorsalis twenty four and twenty one fragments were amplified ranging from 150bp-2000bp, respectively. Four out of ten primers were monomorphic. Mean heterozygosity in B. cucurbitae and B. dorsalis was 0.119 and 0.093, respectively. Nei’s genetic identity value (0.831), calculated using Tools For Population Genetic Analysis (TFPGA) software, reveals very close genetic similarity between the two species.

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Published

31.03.2011

How to Cite

R. SINGH, N. BAJPAI, & R. R. TEWARI. (2011). Genetic Characterization Of Bactrocera (Dacus) Flies (Diptera: Tephritidae) Based On RAPD-PCR. International Journal of Pharma and Bio Sciences, 2(2`), 499–504. Retrieved from https://ijpbs.net/index.php/journal/article/view/856

Issue

Volume 2, Issue 2, 2011

Section

Research Articles

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