Rapid And Efficient Purification Method Of Phospholipase A2 From Acanthaster Planci

Authors

  • IMELDA KRISANTA ENDA SAVITRI Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia 2 Department of Aquatic Product Technology, Faculty of Fishery, Pattimura University, Jl. Ir. Martinus Putuhena, Kampus UnPatti, Poka, Ambon 97233, Indonesia
  • FERA IBRAHIM Research and Healthcare Service Center on Virology and Cancer Pathobiology, Faculty of Medicine, Universitas Indonesia, Kampus UI, Salemba, Jakarta 10430, Indonesia
  • MUHAMAD SAHLAN Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia
  • ANONDHO WIJANARKO Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia

Keywords:

Starfish, Simple method, Purification, Phospholipase A2, yield and activity

Abstract

Rapid and efficient purification method of the phopholipase A2 (PLA2) from the crown-of-thorns starfish Acanthaster planci was done. It was developed without using separation column and taken less time around one day purified. Compare to common procedure that was done by purification measurement using several columns chromatography and was known taken several days, this purification method is more simplify. This purification method use only 30 minutes heat treatment on 60oC and then is fractionated by 20% saturated ammonium sulfate precipitation. The protein complex was analyzed by one dimensional sodium dodecylsulfate polyacrylamide gel electrophoresis.  PLA2 is obtained an excellent overall yield with a final enzyme activity was similar to result of common procedure using four columns chromatography.  

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Published

31.03.2011

How to Cite

IMELDA KRISANTA ENDA SAVITRI, FERA IBRAHIM, MUHAMAD SAHLAN, & ANONDHO WIJANARKO. (2011). Rapid And Efficient Purification Method Of Phospholipase A2 From Acanthaster Planci. International Journal of Pharma and Bio Sciences, 2(2`), 402–407. Retrieved from https://ijpbs.net/index.php/journal/article/view/842

Issue

Volume 2, Issue 2, 2011

Section

Research Articles

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