Production Of Chitinase By Streptomyces Hygroscopicus VMCH2 By Optimisation Of Cultural Conditions

Authors

  • PRIYA.C.S Department of Biotechnology, Vels University, Velan Nagar, Pallavaram, Chennai, Tamilnadu 600 117
  • JAGANNATHAN.N Department of Biotechnology, Vels University, Velan Nagar, Pallavaram, Chennai, Tamilnadu 600 117
  • KALAICHELVAN.P.T Center of Advanced Studies in Botany, University of madras, Guindy Campus, Chennai 600025.

Keywords:

Streptomyces hygroscopicus, chitinolytic bacteria, chitinase cultural conditions.

Abstract

A total of 10 chitinolytic bacteria were isolated from 35 soil samples collected from different crop fields in Tamilnadu state, India. Among them, a strain designated as VMCH2 which produced highest chitinolytic activity in primary and secondary screening in colloidal chitin agar was selected and later identified as Sterptomyces hygroscopicus. The maximum chitinase production was observed in CCMB amended with 0.2% colloidal chitin at pH 7.0 and 35° C after 8 days of incubation. Under this optimized growth conditions, Streptomyces hygroscopicus - VMCH2 produced a total chitinase activity of 28. 09 units / ml against only 9.36 units / ml in the initial production medium stage.

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Published

31.03.2011

How to Cite

PRIYA.C.S, JAGANNATHAN.N, & KALAICHELVAN.P.T. (2011). Production Of Chitinase By Streptomyces Hygroscopicus VMCH2 By Optimisation Of Cultural Conditions. International Journal of Pharma and Bio Sciences, 2(2`), 211–220. Retrieved from https://ijpbs.net/index.php/journal/article/view/820

Issue

Volume 2, Issue 2, 2011

Section

Research Articles

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