Estimation of Two Bioactive Compounds From Azadiracta Indica A. Juss. Leaves Using HPLC

Authors

  • WILLY SHAH Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai-400 019.
  • NILAN RANE Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai-400 019.
  • M.B.KEKARE Department of Chemistry, Kirti College, Dadar, Mumbai-400 028.
  • VIKAS VAIDYA Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai-400 019.

Keywords:

HPLC, Rutin, Quercetin, Azadiracta indica A.Juss.

Abstract

Sensitive, simple, and accurate high-performance liquid chromatographic method has been established for determination of rutin and quercetin both simultaneously in Azadiracta indica A.Juss. leaf powder. The chromatographic separation was performed on Phenomenex C18 column (250 x 4.6, 5 μm) with a 60:40 v/v mixture of methanol and 0.1% O-phosphoric acid in water at the flow rate of 1.2 mL/min and detection at 258 nm, with a run time of 10.0 min. The developed method was then validated using statistical analysis. This will help in qualitative analysis of plant material using fingerprint pattern and quantitative analysis of rutin and quercetin.

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Published

30.06.2010

How to Cite

WILLY SHAH, NILAN RANE, M.B.KEKARE, & VIKAS VAIDYA. (2010). Estimation of Two Bioactive Compounds From Azadiracta Indica A. Juss. Leaves Using HPLC. International Journal of Pharma and Bio Sciences, 1(2), 1–7. Retrieved from https://ijpbs.net/index.php/journal/article/view/78

Issue

Volume 1, Issue 2, 2010

Section

Research Articles