Simultaneous Estimation of Linagliptin and Ascorbic Acid using UV Spectrophotometry: Development, Validation and Stability-Indicating Method
Keywords:
Simultaneous estimation, UV spectroscopy, Stability study, Linagliptin, Ascorbic acidAbstract
The design of pharmaceutical products using a dual or combination drug delivery method may complicate the simultaneous identification and detection of both drugs at the same time. The most straightforward, accurate, and reliable method among the many that are attainable is UV spectroscopy. We have effectively developed and validated the UV Spectroscopy method for the simultaneous quantification of Linagliptin and Ascorbic acid.The recovery percentages of each drug at 80%, 100%, and 120% of the established simultaneous equation were determined to be 99.6% to 95.8%, 99.3% to 100.7%, and 99.4% to 99.7% for LGT and AA, respectively. The percent relative standard deviation of the LGT and AA combination, during intra-day analysis, was determined and found to be 0.223% at λ1 and 0.715% at λ2, respectively. In the inter-day investigation, the % RSD was found to be 0.220% at λ1 and 0.852% at λ2, respectively. Our analysis indicates that our methodology is precise and accurate. We have further validated our technique using forced degradation testing in a stability study. Both drugs were shown to degrade under oxidative, alkaline, and acidic environments. Studies on thermal and photolytic deterioration were also performed. The data indicate that our simultaneous estimation UV Spectroscopy method is reliable and may facilitate new research opportunities.
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