Isolation and Study of Structural Analogue of Bioactive Chemical Dibutyl Phthalate from Leaves of Pithecellobium Dulce Plant

Abstract

Pithecellobiumdulce is a species of the family fabaceae. The methanolic extract of leaves was obtained by a Soxhlet extractor followed by concentration in rotary evaporator. Separation of bioactive chemicals was carried out by column chromatography and thin layer chromatoghraphy while studies by GC-MS which shows presence of six major fractions were collected by eluting the column with ethyl acetate: formic acid: acetic acid: water  at 100:11:11:26 proportions. Major fractions arePhytol, Dibutylphthalate, Anthracene, 9(3butenyl), 1,3Docosenamide, 3, 6, 9Triethyl,3 6, 9trimethyl Formic acid. The major component was subjected to further purificationby using preparative thin layer chromatography. The bioactive component recovered from TLCanalyses by GC-MS &NMR. The¹H-NMR spectrum shows the peak at δ 4.08- 4.13 corresponds to the protons of the cyclic ring of aromatic hydrogen appear as multiplet. The CH₂-O nearer to phthalate group gives signal near δ 2.11-2.16 ppm is appeared as doublet, then butyl groupaliphaticCH₂ gives signal nearδ 1.91-1.96 is appeared as doublet also but δ 1.31-1.28 value showing CH₃ of the di-butyl group. On the basis of above spectral data of GC-MS,¹HNMR& FT- IR assign structure of isolate Pithecellobiumdulce leaves.