Bioanalytical evaluation of pha co-polymer produced by Massilia Spp isolated from Rhizosphere soil

Authors

  • Priyanka Naik Guru Nanak Khalsa College of Arts, Science and commerce, Department of Microbiology, Nathalal Parekh Marg, Matunga, Mumbai -400019, Maharashtra, India.
  • Vaijayanti Ranade Guru Nanak Khalsa College of Arts, Science and commerce, Department of Microbiology, Nathalal Parekh Marg, Matunga, Mumbai -400019, Maharashtra, India.

Keywords:

Polyhydroxyalkanoate, co-polymer, Massilia spp, Confocal laser scanning microscope, 3- Hydroxybutyric acid, Octadecanoic acid.

Abstract

Polyhydroxyalkanotes are naturally occurring biodegradable polymers. The aim of the present study was to optimize and characterize PHA extracted from a rhizosphere soil isolate. 16S rRNA sequencing revealed that isolate belongs to the genus Massilia. Confocal laser scanning microscopy revealed the presence of bright orange colored fluorescent granules when grown under nitrogen limiting conditions and stained with Nile red dye. Present isolate was known to produce highest amount of  PHA when supplemented with maltose as a carbon source and NH4HPO4 as a nitrogen source. After optimization, isolate produced 2.0g/l of PHA. FTIR spectrum of PHA revealed the presence of C=O, -OH and C-O bonding.  Td of extracted polymer was found to be at 2810C.  NMR and GC-MS spectrum revealed that it is a co-polymer of 3-hydroxybutyric acid (3HB) and 9, 19-dihydroxy octadecanoic acid (HOD).

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Published

31.03.2020

How to Cite

Priyanka Naik, & Vaijayanti Ranade. (2020). Bioanalytical evaluation of pha co-polymer produced by Massilia Spp isolated from Rhizosphere soil. International Journal of Pharma and Bio Sciences, 11(1), 106–113. Retrieved from https://ijpbs.net/index.php/journal/article/view/6797

Issue

Volume 11, Issue 1, 2020

Section

Research Articles

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