Assay Of Rifampicin In Pharmaceutical Formulation: Comparison Of Spectrophotometric And Microbiological Methods

Authors

  • SARBJIT SINGH JHAMB Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, Punjab – 160062, India
  • RAMAN PREET SINGH Department of Pharmacology, Akal College of Pharmacy and Technical Education,Mastuana Sahib, Sangrur, Punjab – 148 001, India †Present address: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, Punjab – 160062, India ** Both authors contributed equally

Keywords:

Rifampicin, Micrococcus luteus, microbiological assay, pharmaceutical formulations, fixed dose combination

Abstract

Micrococcus luteus was employed for the assay of rifampicin in fixed-dose combinations (FDCs) of anti-tubercular drugs containing rifampicin, isoniazid, pyrazinamide and ethambutal. The microbiological assay was compared with spectrophotometric assay for estimation of rifampicin in the FDCs as well as in dissolution studies of the FDCs. The microbiological method was found to be more sensitive and accurate as compared to spectrophotometric assay and did not show any interference in the presence of other anti-tubercular drugs. The limits of detection and quantification for microbiological method were 0.1 µg/ml and 0.64 µg/ml, respectively.

Downloads

Published

31.12.2010

How to Cite

SARBJIT SINGH JHAMB, & RAMAN PREET SINGH. (2010). Assay Of Rifampicin In Pharmaceutical Formulation: Comparison Of Spectrophotometric And Microbiological Methods. International Journal of Pharma and Bio Sciences, 1(4), 294–302. Retrieved from https://ijpbs.net/index.php/journal/article/view/674

Issue

Volume 1, Issue 4, 2010

Section

Research Articles