Antimicrobial susceptibility pattern of urinary isolates of Klebsiella Pneumoniae in a tertiary care hospital

Abstract

The prevalence of multidrug resistant organism is a major obstacle in the management of urinary tract infections (UTI). Among the bacterial species, Klebsiella pneumoniae is the second most common organism causing UTI following Escherichia Coli. The expression of extended spectrum β-lactamase by this virulent bacterium is now a major concern in nosocomial infectionsas it can show resistance to other classes of antibiotics like aminoglycosides and fluoroquinolones. The aim of the present study is to assess the antimicrobial susceptibility pattern of K. pneumoniae bacteria isolated from urinary isolates in a Tertiary Care Hospital, Chennai, India. The identification and antibiotic susceptibility testing of K. pneumoniae urinary isolates were performed by Vitek 2 systems version 7.0 as per CLSI guidelines. Paired t- test was performed to calculate the statistical significance between susceptible and resistant groups. K. pneumoniae urinary isolates showed a high resistance to cefuroxime (36.80%), followed by ceftriaxone (26.38%), nalidixic acid (23.61%), cotrimoxazole (22.91%), amoxicillin (20.83%), ciprofloxacin (20.13%) and gentamicin (17.36%).  Few isolates exhibited multi drug resistance (4.2%). All the isolates were susceptible to colistin. . A statistical significant mean difference was observed between the susceptible and resistant groups. (p=0.007). Therapeutic options are limited for multidrug resistant isolates.  . Molecular characterization of highly resistant and moderately resistant isolates will throw more light in reporting the resistance patterns in a tertiary care hospital setting.