In vitro antibiofilm property of streptomyces parvulus against biofilm forming candida albicans

Abstract

Biofilm formation is one of the most important phenomenon which enable microorganisms to establish infection in a host. Biofilm formation by Candida has been the substantial reason for its persistent infection and antibiotic resistance. Nowadays, research has been focused on identifying an alternative source for preventing the establishment of biofilms. Actinomycetes have an immense potential to synthesize diverse bioactive compounds with multitude of activity. The present study investigates the antibiofilm property of Actinomycetes sp. isolated from soil   against biofilm forming C. albicans. Soils samples were collected from Anaikatti hills of Coimbatore, Tamil Nadu, and India. The soil samples were then subjected to serial dilution and plated on SCN media. The potential pigment producing colonies were screened, isolated, and characterized by morphological, biochemical and molecular techniques. Then pigment extraction was performed and the extracted pigments were evaluated for the antibiofilm activity. Minimum Inhibitory Concentration (MIC) of the purified pigment extract of isolates were evaluated. The results of the present study demonstrated that about 32 isolates were obtained from the soil samples and six pigment producing isolates were then tested for antibiofilm activity against C. albicans in which the pigment extract of isolate CBT-BU1 showed >50% biofilm reduction. The Minimum Inhibitory Concentration was found to be 100 µg/ml and the Biofilm Inhibitory Concentration of the tested isolate was found to be 50µg/ml against C. albicans. Molecular characterization of the isolate revealed that the isolate was Streptomyces parvulus. Further, the methanol pigment extract of CBT-BU1 was characterized and analysed using TLC, HPLC and FTIR which revealed the presence of bioactive principle compounds among which, carotenoid was predominantly present at higher concentration which elucidated the antibiofilm activity against test pathogen.