High performance liquid chromatographic method for quantitation of apigenin from dried root powder of Gmelina arborea Linn

Authors

  • SHREEDA ADHYAPAK Department of Chemistry, Ramnarain Ruia College, Mumbai – 400019, India
  • Dr. VIDYA DIGHE Department of Chemistry, Ramnarain Ruia College, Mumbai – 400019, India
  • DHANASHRI MESTRY Department of Chemistry, Ramnarain Ruia College, Mumbai – 400019, India
  • NEETA SHAMBHU Department of Chemistry, Ramnarain Ruia College, Mumbai – 400019, India

Keywords:

Column liquid chromatography, Apigenin, Gmelina arborea Linn., Densitometry

Abstract

A simple fast and precise reverse phase high performance liquid chromatographic method is developed for the quantitative determination of apigenin, a flavonoid from dried root powder of Gmelina arborea Linn. Apigenin was extracted from root powder of Gmelina arborea Linn. by using warm methanol as the extracting solvent. The quantization of apigenin was carried out on an Inertsil ODS-3V-C18, (25cm x 4.6 mm i.d., 5µm) column, using acetonitrile and distilled water in volume ratio of (45:55), as the mobile phase. The detection of the standard was carried out at 340 nm, using UV-Visible detector, which is reported to be the wavelength of maximum absorption of the standard apigenin.The proposed HPLC method was validated and applied for the quantitative determination of apigenin from Gemlina arborea Linn 

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Published

31.03.2011

How to Cite

SHREEDA ADHYAPAK, Dr. VIDYA DIGHE, DHANASHRI MESTRY, & NEETA SHAMBHU. (2011). High performance liquid chromatographic method for quantitation of apigenin from dried root powder of Gmelina arborea Linn. International Journal of Pharma and Bio Sciences, 2(1), 742–749. Retrieved from https://ijpbs.net/index.php/journal/article/view/634

Issue

Volume 2, Issue 1, 2011

Section

Research Articles

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