Biochemical analysis on some fungal isolates against their growth and sporulation

Abstract

Several biochemical parameters represent distinguishable vital consequences on fungal physiology and morphological changes. The effect of various culture conditions supplemented with different  biochemicals, was examined to identify optimal and tolerated conditions for fungal growth and sporulation. In the present study, the effect of Sodium chloride (NaCl), Indole-Acetic acid (IAA) and Indole-Buteric acid (IBA) were analyzed on five different fungal isolates namely, Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum gypseum, Microsporum canis and Chrysosporium tropicum. Analysis revealed that the remarkable growth and sporulation were identified at between 0.5 % to 3% NaCl concentrations within T. mentagrophytes, T. rubrum, M. gypseum and C. tropicum but retarded response was seen over exceeding 3% NaCl concentration. Moreover, T. mentagrophytes revealed remarkable response against 0.5 to 10 ppm IAA and 1 to 5 ppm (IBA) concentration. Similarly, T. rubrum reflected inducible changes at between 0.5 to 3 ppm concentration of both IAA and IBA. Thus, each fungus revealed a characteristic differentiation in their morphological features against the biochemical tolerance and offered a possibility for recognition and categorization of these fungi.