α- amylase and α-glucosidase inhibitory activity of ethyl acetate extract of aspergillus tamarii isolated from justicia beddomei

Abstract

Fungal endophytes attain importance due to the innumerable diverse and potent activity of their secondary metabolites. The present study was carried out to screen the anti diabetic activity of the fungal endophyte Aspergillus tamarii isolated from Justicia beddomei. Bioactive metabolites were extracted with ethyl acetate and the qualitative phytochemical analysis established the presence of phenol, flavonoids and alkaloids. α amylase and α glucosidase inhibitory activity of the ethyl acetate extract was assessedby biochemical assays with Acarbose as standard. α – amylase inhibitory activity was found to be 83.26% at a concentration of 1000µg/ml. A maximum inhibition of 91.79%was achieved at a concentration of 1000µg/ml while standard acarbose inhibition was about 97% for α – glucosidase inhibitory activity. The inhibitory activity can be attributed to the phytochemicals present in the ethyl acetate extract of the endophyte. Further studies need the purification of the compound and the study on cell lines.