Phytochemical analysis and antibacterial activity of mangifera indica l and piper betle

Abstract

The leaves of Mangifera indica and Piper betle are known for their antioxidant and antimicrobial properties. In the present study, the phytochemical constituents of the leaves of Mangifera indica and Piper betle were analyzed and their antibacterial properties were tested against bacteria isolated from dental caries. Staphylococcus aureus (ASDV5,6,7,12), Staphylococcus epidermidis (ASDV 4, 10), Streptococcus mutans (ASDV 2, 3, 9) were isolated on Mannitol salt agar and blood agar from caries tooth scum samples. All the isolates of S. aureus, S. epidermidis and Streptococcus mutans were sensitive to Ciprofloxacin, Azithromycin, Tetracycline and resistant to Clotrimazole, Methicillin. Leaf extracts of Mangifera indica and Piper betle were prepared using various solvents and tested against the bacterial isolates. The phytochemical analysis revealed the presence of tannins, flavonoids, alkaloids, terpenoids, glycosides, carbohydratesË saponins and resins. The antibacterial  activity of  the  leaf  extract  of  M. indica  showed a  zone  of  inhibition  ranging from 10 to 20 mm at a concentration of 50, 100 µg whereas the antibacterial activity of the leaf extract of P. betle showed a zone of inhibition ranging from 10 to 25mm at the same concentration against S. aureus, S. epidermidis and Streptococcus mutans. The findings of the study indicate a propensity of these plants for drug development.