APPLICATION OF SOLUBLE AND IMMOBILIZED α - GALACTOSIDASE FROM ACINETOBACTER SP FOR DEGRADATION OF GALACTO-OLIGOSACCHARIDES IN COWPEAS

Authors

  • SIRISHA E Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, Telangana, India
  • RANJIT P Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, Telangana, India
  • LAKSHMI NARASU M Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, Telangana, India

DOI:

https://doi.org/10.22376/ijpbs.2017.8.1.b603-609

Keywords:

k-carrageenan, immobilization, galacto-oligosaccharides, α-galactosidase, cowpeas, flatulence

Abstract

α-galactosidase purified from Acinetobacter sp. was immobilized in four different matrices- Calcium
alginate, Gelatin, Agarose and k-Carrageenan. On comparative analysis, k-Carrageenan immobilized
enzyme showed highest immobilization yield of 68% and storage stability for 30 days. Gelatin showed an
immobilization yield of 57%. Soluble enzyme led to decrease of 84% in raffinose and 76% in stachyose
concentration in cowpeas whereas k-Carrageenan immobilized enzyme led to decrease of 72% of
raffinose and 54% of stachyose thereby decreasing the flatulence to greater extent.

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Published

31.03.2017

How to Cite

SIRISHA E, RANJIT P, & LAKSHMI NARASU M. (2017). APPLICATION OF SOLUBLE AND IMMOBILIZED α - GALACTOSIDASE FROM ACINETOBACTER SP FOR DEGRADATION OF GALACTO-OLIGOSACCHARIDES IN COWPEAS. International Journal of Pharma and Bio Sciences, 8(1), 603–609. https://doi.org/10.22376/ijpbs.2017.8.1.b603-609

Issue

Volume 8, Issue 1, 2017

Section

Research Articles

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