Antibiotic resistance profile and detection of metallo – beta lactamase in pseudomonas aeruginosa Isolates from clinical specimens in A tertiary care hospital

Abstract

This study aims to analyse the prevalence, antibiotic resistance pattern and detection of metallo beta lactamase in Pseudomonas aeruginosa isolates from clinical specimens. A total of 378 Pseudomonas aeruginosa isolates were isolated over a period of one year. Antibiotic susceptibility testing was done using Kirby Bauer disc diffusion method following standard guidelines. Inpatient samples contributed to majority of the isolates, proving its nosocomial origin. On the whole there was an increase in resistance percentage in the inpatient isolates reiterating the fact that selection pressure and antibiotic resistance are directly proportional to the use of antibiotics. Highest resistance was noted for Ceftazidime (51.85%). 19 isolates showed carbapenem resistance out of which 11 isolates were Metallo-beta-lactamses producers. Continuous monitoring of antibiogram, revising antibiotic policies periodically, antibiotic stewardship and infection control measures should help us win the war against one of the wisest unicellular bacteria.