Evaluation of anti-diabetic effect of ethanolic extract Of andrographis echioides by alpha glucosidase and alpha amylase assay

Abstract

Andrographis echioides ,otherwise called as false water willow is a medicinal plant which was used as the remedy for curing fever,headache,constipation etc.This plant is mainly found in countries like India and Srilanka. The above study involves evaluation of  anti diabetic activity of ethanolic extract of Andrographis echioides plant (gopuram Thaangi) using alpha glucosidase assay and alpha amylase assay. The collected whole plant material was extracted using ethanol solvent and was further concentrated under reduced pressure. This was subjected to an in vitro alpha glucosidase and alpha amylase inhibition activity for anti diabetic effect whose absorbance was measured using spectrophotometery. Acarbose, an approved antidiabetic drug  was used as a positive control. Alpha glucosidase inhibition activity of ethanolic extract of Andrographis echioides was 75.7% with 50% inhibitory concentration (IC50) of  500 mcg/ml and that of Alpha amylase inhibition activity was 66.6% with IC50 of around 500 mcg/ml.  Acarbose (reference) exhibited 94.6 % inhibition with IC50 of  250 mcg/ml. The assay results show that the “Andrographis echioides” has inhibitory activity on alpha glucosidase enzyme and alpha amylase enzyme. The alpha glucosidase inhibition is more compared to alpha amylase inhibition but  their antiglucosidase and antiamylase activity is less when compared to the control drug Acarbose.