Aqueous two phase purification of xylanase obtained from a bacterial isolate

Authors

  • GIRISHA MALHOTRA Department of Biotechnology,Faculty of Engineering and Technology, Manav Rachna International University
  • RAJKUMARI JESHREENA Department of Biotechnology,Faculty of Engineering and Technology, Manav Rachna International University
  • Dr. SHILPA S CHAPADGAONKAR Department of Biotechnology,Faculty of Engineering and Technology, Manav Rachna International University

Keywords:

Xylanase, Aqueous two phase systems, ATPS, Lignocellulose degradation, Enzyme purification

Abstract

The aim of this work was to extract and to purify xylanase produced by the bacillus strain from the crude fermentation broth using aqueous two phase system (ATPS). Xylanase is an important industrial enzyme that finds use in several industries like paper and pulp, textiles, food and feed and production of bioethanol from agri-residues. There is a huge demand for a cost effective xylanase in the industry. The xylanase was extracted by partitioning in ATPS composed of phosphate and polyethylene glycol (PEG). The technique resulted in 1.35 fold purification. The specific activity of the xylanase obtained was 6.27mu/mg.

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Published

31.12.2016

How to Cite

GIRISHA MALHOTRA, RAJKUMARI JESHREENA, & Dr. SHILPA S CHAPADGAONKAR. (2016). Aqueous two phase purification of xylanase obtained from a bacterial isolate. International Journal of Pharma and Bio Sciences, 7(4), 305–308. Retrieved from https://ijpbs.net/index.php/journal/article/view/5473

Issue

Volume 7, Issue 4, 2016

Section

Research Articles

Categories

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.