ANALYSIS OF PHYTOCHEMICAL AND ANTI-MICROBIAL ACTIVITY ON VETIVERIA ZIZANIOIDES ETHANOLIC EXTRACT FOR HEALTHCARE APPLICATIONS

Abstract

One of the abundant sources of strong natural fiber from the plant kingdom is Vetiveria Zizanioides and it has numerous properties such as antimicrobial and inherently filled with good aroma. The soxhlet hot extraction method was used for extracting vetiver using ethanol. The extract was tested under standard qualitative phytochemical screening method and the test results revealed the presence of  phytochemical constituents such as carbohydrates, proteins, steroids, alkaloids, flavanoids, phenols and tannins. The vetiver extract(100µg/ml) was also assessed for antibacterial activity using agar well diffusion standard test method over gram positive bacterial pathogens such as Staphylococcus aureus, Klebsiella pneumoniae and gram negative bacterial pathogens Pseudomonas auruginosa, Escherichia coli and the antifungal activity were assessed against Aspergillus brasiliensis and Aspergillus fumigates. The measured zone of inhibition results showed good antibacterial activity against gram positive bacteria than gram negative bacteria. The antifungal test also exhibits better results in Aspergillus brasiliensis than Aspergillus fumigates.