ROLE OF THIAMINE AND ITS MOIETIES IN GROWTH RATE OF DIATOM SP.

Abstract

Diatom sp isolated from the coastal water around Chennai, and Tamilnadu was made as
unialgal and investigated for growth. The culture is grown aseptically in F/2 medium under
constant temperature and light. This study focus on the influence of thiamine and its moieties
in Diatom sp. Thiazole and pyrimidine are two thiamine moieties were used as vitamin
source in diatom culture medium separately. Three diatom cultures were used for this study.
All diatom cultures were grown separately in (A) Medium.1 -F/2 medium with thiamine.
(B)Medium.2 -F/2 medium with thiazole. (C)Medium.3 -F/2 medium with pyrimidine. The
result shows that Diatom Navicula sp reaches maximum growth rate thiazole medium and
Diatom Cymbella sp reaches maximum growth rate pyrimidine medium and Diatom
Cyclotella sp. reaches maximum growth rate of both moiety of thiamine control medium.
From this research we conclude that Navicula sp need only thiazole and Cymbella sp need
only pyrimidine and Cyclotella sp need both thiamine moieties for their normal growth.