STUDY OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF PHOTOACTIVATED THIOPHENE DERIVATIVES FROM THE ROOTS OF TAGETES ERECTA

Abstract

Photodynamic antimicrobial chemotherapy (PACT) comprises of treatment with combination of a photosensitizing drug and visible light which causes discriminating destruction of microbes. The thiophene derivative (α- Tertheinyl) isolated from roots of Tagetes erecta exhibits such photodynamic activity. α-terthienyl generates reactive oxygen species upon irradiation with near ultraviolet light which exhibit cell destruction. With this objective the detailed photodynamic antibacterial and antifungal activity was planned for the plant Tagetes erecta. The study comprises of microscopic characterization, phytochemical investigation, optimization of extraction condition for roots of Tagetes erecta by Supercritical fluid extraction techniques. The extracted volatile oil containing thiophene derivative which has photosensitizing property was subjected for their photodynamic antimicrobial activity by dilution and pour plate method. The result revealed that the extract of roots of Tagetes erecta containing thiophene derivatives showed good photosensitizer antibacterial and antifungal activity at concentration of 300µg and the observed activity shows 35% potentiating when expose to light.