ANTIBACTERIAL ACTIVITY OF ACTINOMYCETES ISOLATED FROM CAPSICUM ANNUUM L.

Abstract

A total of 72 actinomycetes strains were isolated from Capsicum annuum L. rhizosphere soils of Warangal, Khammam, Karimnagar and Mahabubnagar from Telangana State, India. Among these 25 showed antifungal activity against Colletotrichum capsici and Fusarium oxysporum. Among these 25 strains, three (OUA17, OUA18 and OUA27) were grown in starch casein broth for 6 days, centrifuged and powdered samples were extracted by rotary evaporator and were screened for antibacterial activity against bacterial pathogens viz., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Klebsiella pneumoniae. All the three strains showed growth inhibition against these pathogenic bacteria. Among the three strains OUA27 showed highest inhibition against E. coli. These actinomycetes strains were identified by 16S rRNA sequence as Pseudonocardia sp. OUA17TAVKSP, Streptomyces sp. OUA18TAVKSP and Streptomyces graminearus OUA27TAVKSP, deposited in NCBI database and the accession number given were KP013381, KP013382 and KP013383 respectively. The phylogenetic tree was constructed by using phylogeny.fr online phylogeny programs.