GENETIC DIVERSITY AMONG SALMONELLA SPECIES ISOLATED FROM SOIL AND SEWAGE SAMPLES IN NANDED, INDIA

Abstract

A total of fourty four salmonella spp. isolates randomly from sewage and soil samples were included for the genetic closeness and diversity study using Pulsed Field Gel Electrophoresis (PFGE) and Bionumerics. Xbal restriction enzyme band pattern was produced and the same was analyzed using Applied Maths software. Standard ISO method was used for the isolation of the bacteria from the samples. The suspect isolates were confirmed using RT PCR technique for fast processing and reliability of the result. 24-27 hours standard protocol for salmonella spp. from World Health Organization (WHO) was used for the PFGE run setup. All 44 isolates produced clear finger print pattern and out of which 33 were grouped in to one cluster (Cluster A) and the rest 13 into Cluster B. The cluster A contains 4 groups which exhibited 100% similarity in their genetic structure, out of which the group one and two contains 6 and 3 isolates, while group three and four contained 2 isolates each.  Cluster B had only two groups with 100% similarity and the first group with 8 and the later with 2 isolates. There were 18 isolates which was not genetically similar to each other in cluster A, and 3 in cluster B. From the result it was able to conclude the spread of salmonella spp. in sewage and soil across various area of Nanded district.