Rdna ITS1 SEQUENCE ANALYSIS BASED GENOTOXICITY ASSESSMENT OF DICOFOL BY USING CULEX QUINQUEFASCIATUS (DIPTERA: CULICIDAE)

Abstract

Present research work is attributed to mutagenicity assessment of dicofol by using Culex quinquefasciatus, exposed to LC₂₀ for 24 hours. To achieve present targets, rDNA ITS1 sequence of treated as well as control individuals was amplified by using specific forward and reverse primers with sequences FR 5´-C C T T T G T A C A C A C C G C C C G T-3´  and RP 5´-G T T C A T G T G T C C T G C A G T T C A C-3´. Sequence alignment of treated and control stocks was done by the Clustal W software programme, subsequently it was observed that dicofol induced a total of 334 alterations in rDNA ITS1 sequence, which included 33 deletions, 49 additions, 120 transitions and 132 transversions. Futhermore, dicofol caused maximum deletions and additions of cytosine base.