Optimization Of Callus And Cell Suspension Culture Of Capsicum Annum L

Abstract

Tissue culture optimization may play an important role to optimize the cell growth of chilly. Plant produced through in vitro techniques are true to type and are free from diseases. The present investigations were carried out to standardize surface sterilization of explants, suitable explants type for culture establishment, growth regulators for callus formation and to determine cell growth. It was observed that water socked blotting paper gave the maximum seed germination. In all the combination of plant growth regulators the highest number of callus formation was observed in growth regulators 2, 4-D (0.1 µM/L) and BAP (0.1 µM/L) containing MS medium.  Cells grew and multiplied rapidly with a doubling time of 0.72 days. Elongated and divided cells were observed more in 21 days. Fresh weight and PCV% of the cells increased nearly 3 folds with 8.64% and 0.77% mean of growth. In the present study, this method was used to develop a growth curve for chilly suspension culture identifying the onset and duration of the various growth phases, growth rate and doubling time.