ANTIBACTERIAL ACTIVITY OF FLOWER AND STEM EXTRACTS OF CHRYSANTHEMUM CORONARIUM

Abstract

The present study aimed at evaluating in vitro antibacterial activity of flower and stem extracts of Chrysanthemum coronarium in methanolic and acetone solvents by soaking and soxhlet method. The antibacterial activity was evaluated using the agar well diffusion method at four different concentrations (ie 2,3,4 and 5 mg/ml). DMSO was used as a solvent to dissolve the extracts and Ampicillin as a positive control. The extracts were tested against two gram positive (S.aureus and B.subtilis ) and  gram negative bacteria ( P. aeruginosa  and E.coli) .  All the extracts showed a good inhibitory activity against the gram positive bacteria (S.aureus and B.subtilis).  The highest antibacterial activity was shown by methanolic hot extract of C. coronarium stem against B. subtilis (36mm) and the lowest was shown by the acetone cold extract stem against E.coli (13mm) . The antibacterial activity against both type of bacteria indicated broad spectrum of secondary metabolites. Hence it can be used as a therapeutic drug to inhibit bacterial pathogens.