Isolation and PCR Amplification of Genomic DNA from Dry Leaf Samples of Sugarcane

Authors

  • A. VAZE Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari, Pune (Maharashtra), 412307, India.
  • G. NERKAR
  • M. PAGARIYA
  • R. M. DEVARUMATH Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari, Pune (Maharashtra), 412307, India. Visiting fellow, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, NSW 2480, Australia
  • D. THEERTHA PRASAD Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari, Pune (Maharashtra), 412307, India.

Keywords:

Sugarcane, genomic DNA, PCR, RAPD, ISSR

Abstract

Efficient method for isolation of high quality genomic DNA is the first step in the development of DNA based markers for fingerprinting and genetic diversity of crops. We have described a simple and user friendly protocol for extraction of DNA from dried sugarcane leaf samples, which dose not require any use of liquid nitrogen, making it advantageous over other common protocols. The DNA obtained using this procedure were consistently amplifiable, using both RAPD primers as well as ISSR primers, making it most useful in genetic diversity analysis of the sugarcane germplasm. 

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Published

30.06.2010

How to Cite

A. VAZE, G. NERKAR, M. PAGARIYA, R. M. DEVARUMATH, & D. THEERTHA PRASAD. (2010). Isolation and PCR Amplification of Genomic DNA from Dry Leaf Samples of Sugarcane. International Journal of Pharma and Bio Sciences, 1(2), 1–6. Retrieved from https://ijpbs.net/index.php/journal/article/view/443

Issue

Volume 1, Issue 2, 2010

Section

Research Articles