Molecular Characterization of Morphologically Similar Four Pieridae Butterflies (Lepidoptera: Insecta) by RAPD-PCR Technique

Authors

  • ASHISH D. TIPLE Department of Zoology, Entomology Division, RTM Nagpur University Campus, Nagpur 40033, (M. S.) India. Forest Entomology Division, Tropical Forest Research Institute, Jabalpur 482021, (M. P.) India.
  • SONALI V. PADWAD Department of Entomology, Central Institute for Cotton Research, Nagpur 440010, (M. S.) India.
  • VISHAL P. DESHMUKH Departments of Botany, SGB Amravati University, Amravati, 444602 (M. S.) India

Keywords:

Butterflies, Genetic diversity, Molecular characterization, Pieridae, RAPD

Abstract

Molecular characterization of four morphologically similar Pieridae butterflies was carried out using RAPD markers. Total 121 bands were scored with 8 decamer primers, of which 115 were polymorphic while 6 were monomorphic and the percentage of polymorphism was 95.04%. Dendrogram based on average similarity coefficient of 08 primers grouped four Pieridae species into two distinct clusters. Cluster-I comprised of 3 species viz. Eurema laeta, E. hecabe and E. blanda, while cluster-II consisted of E. andersonii. Cluster-I further subdivided into two sub-clusters, sub-cluster-I consisting of only Eurema laeta and sub-cluster-II comprised of E. hecabe and E. blanda. Genetic similarities between these four species were discussed on the basis of morphological characters and RAPD data. 

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Published

30.06.2010

How to Cite

ASHISH D. TIPLE, SONALI V. PADWAD, & VISHAL P. DESHMUKH. (2010). Molecular Characterization of Morphologically Similar Four Pieridae Butterflies (Lepidoptera: Insecta) by RAPD-PCR Technique. International Journal of Pharma and Bio Sciences, 1(2), 1–7. Retrieved from https://ijpbs.net/index.php/journal/article/view/441

Issue

Volume 1, Issue 2, 2010

Section

Research Articles