ANTI-LEUKEMIC STUDY OF ETHANOLIC EXTRACT OF SARGASSUM ILICIFOLIUM IN HL-60 CELL LINE INDUCED SWISS ALBINO MICE

Abstract

This study was conceived to screen the anti-leukemic activity of Ethanolic extract of Sargassum ilicifolium in HL-60 cell line induced Swiss albino mice. In order to achieve this goal, the Anti-Leukemic activity of 80% ethanolic extract (200 mg/kg) body weight of Sargassum ilicifolium was evaluated against HL-60 tumor cell lines in mice. After the 5th day of tumor inoculation, the extract was administered daily for 14 days. Mice were sacrificed for observation of antitumor activity. The effect of Sargassum ilicifolium on the body weight of HL-60 bearing mice and simultaneous alterations in the hematological profile and liver biochemical parameters (lipid peroxidation, GSH, SOD and Catalase) were estimated. The Sargassum ilicifolium treated groups showed that the decrease in body weight of HL-60 bearing mice and the hematological profile reverted towards normal levels in extract treated mice. Treatment with ethanolic extract of Sargassum ilicifolium restored the serum biochemical parameters towards normal levels and decreased the levels of lipid peroxidation and increased the levels of reduced glutathione, SOD and Catalase. The ethanolic extract of 80% w/v Sargassum ilicifolium exhibited antitumor effect by modulating lipid peroxidation and augmenting antioxidant defense system in HL-60 cell line induced mice.