OPTIMIZATION OF MEDIA CONSTITUENTS FOR THE PRODUCTION OF SUBTILISIN FROM BACILLUS SUBTILIS USING RESPONSE SURFACE METHODOLOGY

Abstract

Optimization is used to maximize product yield in the pharmaceutical industry, increase high product quality in food production and amplify the degradation process in environmental biotechnology. Statistical analysis proved to be a useful and powerful tool in developing optimum fermentation conditions. Subtilisin is an extracellular alkaline serine protease secreted by members of the genus Bacillus. It catalyzes the hydrolysis of proteins, peptide amides and has a high substrate specificity to fibrin. It is mainly used in the development of quality detergents and also used household cleaning products to remove proteinaceous deposits and stains. A new strain –Bacillus spp that has ability for the production of subtilisin was screened. The production of subtilisin was dependent on the constituents influence on the medium. In this study, Response Surface methodology was designed to study the effect of compound and identified that at yeast extract, peptone and casein at a combination of 6.0 g/l, 5.0 g/l and 10.0 g/l respectively.TheR²=0.991125, adjusted R²=0.983138 and Predicted R²=0.987229 shown in experimental results were in good understanding with the predicted value.