ANALYSIS OF PHYTOCHEMICALS AND ANTIBACTERIAL POTENTIAL OF LONICERA JAPONICA THUNB.

Abstract

Lonicera  japonica (Honeysuckle) is one of the oldest medicinal herbs in known history.  Lonicera  japonica is an ingredient of  herbal tea and has been known thousands of years for its cooling  and detoxification  effects. According to the Natural Medicines Comprehensive Database, Lonicera japonica  is taken orally for digestive disorders, enteritis, dysentery, urinary disorders, diabetes, rheumatoid arthritis, malignant tumours etc. In the present study  ethanolic extract was obtained from leaves and flowers of  Lonicera  japonica and phytochemical analysis was done to screen alkaloids, phenols terpenoids and flavonoids.Antibacterial  activity was carried out by using bacterial strains Staphylococcus aureus  MTCC 3160, Bacillus subtilis MTCC 736, Vibrio cholerae MTCC 3906,  and Salmonella typhi  MTCC 3220.Extracts of leaf and flower in various dilutions were found to have antibacterial activity against tested organisms.Chloroform and  Ethanol leaf extracts of  Lonicera japonica    has shown   high degree of  inhibition (DIZ- 22mm and 22 mm)  against  test organisms. It is found that there was no antibacterial activity exhibited by some concentration. Ethanol and Methanol  flower extracts of  Lonicera  japonica     has shown high  degree of  inhibition (DIZ 21mm-23mm) against  the test organisms and our results supported the usage of Lonicera  japonica in traditional medicine.