IN-VIVO ANTICANCER ACTIVITY OF AQUEOUS EXTRACT OF HOLOPTELEA INTEGRIFOLIA LEAVES AGAINST EHRLICH ASCITES CARCINOMA IN SWISS ALBINO MICE.

Abstract

The main aim of the present study is to evaluate an Antitumor activity of Aqueous extract of leaves of Holoptelea integrifolia (HIAQ) in EAC induced Ascites tumor model: An In-vivo study. The Aqueous extract of leaves of Holoptelea integrifolia was prepared by cold maceration technique. Acute toxicity test was performed using Swiss albino rats before starting the in-vivo anticancer activity, were the MTD was more than 5000mg/kg. The in-vivo Antitumor activity was assessed by administering 250mg and 500mg/kg of Aqueous extract of HI leaves, orally for 9 days and Cisplatin (3.5mg/kg, i.p., Single dose). Various parameters like Change in body weight, Mean Survival Time, Percentage Increase in Life Span, Hematological & Biochemical parameters were assessed. All the parameters were considerably restored towards the normal values. The in-vivo activity of HI 500mg/kg was shown more significant results than 250mg/kg. Hence 500mg/kg was taken for combination study with standard drug Cisplatin.