VIRULENCE AND MOLECULAR CHARACTERIZATION OF AEROMONAS SPP. FROM FISH SAMPLES USING ARDRA TECHNIQUE

Abstract

The detection of Virulence factors of Aeromonas spp. is a key component in determining potential pathogenicity because these factors act Multifuntionally and Multifactorially. In this study, totally 50 carp fish samples were collected, Among these, 8 samples were found to be positive for Aeromonas hydrophila and 13 were found to be positive for Aeromonas salmonicida. Antibiotic resistant patterns were performed by Bauer-Kirby method. Both the species of Aeromonas showed the highest resistance patterns to Amoxicillin, Ampicillin/Cloxacillin, Cefotaxime, and Clarithromycin. This study showed a virulence character of Aeromonas hydrophila (8 isolates) positive for proteolytic (62.5%), lipolytic (75%), and β-Lactamase (100%), hemolytic (31.5%), slime production (100%). In Aeromonas salmonicida (13 isolates) showed positive for proteolytic (61.5%), lipolytic (69.2%), and β-Lactamase (84.6%), and slime production (100%) respectively. Aeromonas spp. isolated from fish samples were genetically characterized by Amplified Ribosomal DNA Restriction Analysis (ARDRA) of 16S rDNA gene by using EcoRI enzyme, previously proposed as a useful tool to identify Aeromonas spp. This present study revealed that the virulence and molecular characterization of Aeromonas spp., is very important to detect the infection in fresh water fish, it should be essential to study the environmental factors, immunosuppressive factors and other adaptability factors of host and pathogen responsible for the establishment of pathogenic state.