AN EFFECTIVE METHOD FOR SHOOT REGENERATION AND CALLUS INDUCTION OF CANAVALIA GLADIATA (JACQ.) DC. AN ECONOMICALLY IMPORTANT LEGUME.

Abstract

A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of Canavalia gladiata (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L – 1.5mg/L) and Indole acetic acid (IAA) (0.5mg/L – 1.5mg/L) for shoot induction. The best shoot proliferation was observed at the concentration of 1.0mg/L of BAP and 1.0 mg/L of IAA from only nodal explants of Canavalia gladiata after 4 weeks of culture. Combination of PGRs (BAP and IAA) was found to be more effective for shoot induction of nodal explants of Canavalia gladiata. For callus regeneration the nodal explants of Canavalia gladiata were used and the MS medium was supplemented with various concentration of α-naphthalene acetic acid (NAA) (0.5mg/L – 3.0mg/L). Highest callus induction was observed at the concentration of 1.5mg/L of NAA, and in this concentration highly desirable creamy white friable callus was observed.